Elastatropin® and Polymorphism
Research has shown that human tropoelastin is isomorphic, with at least 9 slightly different forms of elastin have been identified. Furthermore, different isomorphic compositions have been measured in distinct tissues, including human skin fibroblast, placental and fetal aortal tissues. Each tissue contained a unique tropoelastin mRNA profile. In addition, the presence or absence of a particular exon in any mRNA molecule can be determined through a more detailed analysis of isomorphism.
Protein Genomics believes that due to the complexity shown to date in mRNA isomorphism, the tropoelastin isomorphic composition of an individual may be unique; similar to fingerprints or iris pigmentation. Any population group or even individual could be chosen to serve as a model for a tropoelastin cosmetic composition, their mRNA isolated from skin cells, and the precise isomorphic composition of the tropoelastin mRNA determined. A series of tropoelastin genes would then be synthesized based on the genetic information obtained, introduced into a plant, and a tropoelastin composition modeled after that of the chosen population group or individual produced. A schematic of this process is shown below:
Production of polymorphic tropoelastin. A human population group is sampled (with their permission and the mRNA isolated in step 1. The RNA is amplified in step 2. The relative abundance of each type of elastin mRNA is deduced in step 3. A gene encoding each elastin isomorph is constructed in step 4, and introduced into a host plant. The discrete elastin molecules are produced by transgenic plants in step 5, and the partially purified proteins formulated to match the original relative mRNA abundance. The formulated protein is incorporated into a personal care product in step 6.
The population group serving as a model for the tropoelastin composition could be chosen for one or more characteristics:
- Smooth and beautiful skin
- Celebrity - actor, entertainer, model, sports figure
- Particular ethnic group, location or both
Once the appropriate genes have been synthesized and introduced into a host organism, large quantities of human tropoelastin can be produced by cultivation of transgenic plants. The elastin composition would then be formulated and sold as an ingredient in skin care compositions. This product would provide a source of authentic human tropoelastin with the same characteristics as the material synthesized in the body, be compatible with the biochemistry of the skin, and would be identified directly with the population that served as the source genetic code for the DNA used in the production of the material.